rabbit polyclonal anti biglycan antibody  (Santa Cruz Biotechnology)

Journal: BMC oral health

Article Title: Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.

doi: 10.1186/s12903-024-05211-8

Figure Lengend Snippet: Fig. 4 (a) The mRNA expression of POSTN and biglycan (BGN) related to the extracellular matrix stimulated with transforming growth factor (TGF)-β (10 ng/mL, 24 h). The mean mRNA expression and standard deviation (SD) are shown, *p < 0.05, **p < 0.01, n = 3. (b) Immunofluorescence images of gingival tissue in patients with drug-induced gingival enlargement (DIGE) and idiopathic gingival fibromatosis (IGF). BGN (green), COL1A1 (red), and nuclear (blue). The bar indicates 150 μm. (c) The effect of rhTGM2 on BGN mRNA expression in human gingival fibroblasts (hGF) isolated from a patient with IGF. TGM2 at the indicated dose (ng/mL) was introduced for 24 h. The mean mRNA expression and SD are shown, *p < 0.05, n = 3. (d) Protein levels of SP1 in hGFs isolated from a patient with periodontal disease (PD) and patients with IGF. The mean protein level and SD are shown, **p < 0.01, n = 3. (d) The effect of plicamycin on BGN mRNA expression. Plicamycin was applied at the indicated concentration (mM) for 24 h. The mean mRNA expression and SD are shown, **p < 0.01, n = 3. (f) Schema of the mechanisms underlying IGF development, showing that biglycan upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF

Article Snippet: The primary antibody, rabbit anti-human biglycan (BGN) polyclonal antibody (16409- 1-AP, ProteinTech), rabbit anti-human TGM2 polyclonal antibody (ProteinTech), and goat anti-human type 1 collagen (1310-1, SouthernBiotech, AL, USA) were reacted at 4°C for 24 h. After the reaction was completed and the sections were washed with PBS, goat anti-rabbit IgG H&L (Alexa Fluor 488; Abcam) and chicken anti-goat IgG (H&L) Alexa Fluor 594 (A-21468; Thermo Fisher Scientific) were used as secondary antibodies.

Techniques: Expressing, Standard Deviation, Immunofluorescence, Isolation, Concentration Assay

Journal: Journal of Biological Engineering

Article Title: Proteomic analysis of decellularized mice liver and kidney extracellular matrices

doi: 10.1186/s13036-024-00413-8

Figure Lengend Snippet: Immunohistochemical staining on decellularized and native liver tissues for biglycan, and on decellularized and native kidney tissues for cathepsin Z. The specific detection of biglycan was found to be ubiquitous in the ECM of liver tissues, while cathepsin Z staining was observed in a spot-like pattern in the ECM of kidney tissues. The scale bar equals to 100 µm

Article Snippet: For liver tissues we employed a rabbit polyclonal biglycan antibody diluted to 1:20 (16,409–1-AP, proteintech®, Rosemont, IL, USA) as primary antibody and 1:200 diluted goat anti-rabbit HRP IgG antibody (#ab6721, Abcam) as secondary antibody.

Techniques: Immunohistochemical staining, Staining

Journal: Molecular medicine reports

Article Title: Knockdown of biglycan expression by RNA interference inhibits the proliferation and invasion of, and induces apoptosis in, the HCT116 colon cancer cell line.

doi: 10.3892/mmr.2015.4383

Figure Lengend Snippet: Figure 1. Construction of the stable HCT116 cell line with biglycan down regulation. The (A) mRNA and (B) protein expression levels of biglycan in the shRNA‑biglycan/control‑transfected or non‑transfected cells was detected using reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. (C) The relative protein expression of big lycan was normalized against β‑actin. Each experiment was repeated three times and the data are expressed as the mean ± standard deviation (*P<0.05 or **P<0.01, compared with the shRNA‑control group). sh, short hairpin.

Article Snippet: The membrane was subsequently blocked with 5% non-fat dry milk at room temperature for 1 h, followed by incubation with the following diluted primary antibodies: Rabbit polyclonal anti-biglycan antibody (cat no. sc-33788; 1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), rabbit polyclonal anti-caspase-3 antibody (cat no. wl01992a; 1:1,000), rabbit polyclonal anti-p27 antibody (cat no. wl01769; 1:1,000), rabbit polyclonal anti-cyclin A antibody (cat no. wl01753; 1:200), rabbit polyclonal anti-p21 antibody (cat no. wl0362; 1:100), rabbit polyclonal anti-cyclin D1 antibody (cat no. wl01435a; 1:100) (all from Wanleibio, Shenyang, China), rabbit polyclonal anti-p38 antibody (cat no. sc-7149; 1:100) and anti-phosphorylated-p38 antibody (cat no. sc-101758; 1:100) (both from Abcam, Cambridge, MA, USA) at 4 ̊C overnight.

Techniques: Expressing, Polymerase Chain Reaction, Western Blot, Standard Deviation

Journal: Molecular medicine reports

Article Title: Knockdown of biglycan expression by RNA interference inhibits the proliferation and invasion of, and induces apoptosis in, the HCT116 colon cancer cell line.

doi: 10.3892/mmr.2015.4383

Figure Lengend Snippet: Figure 2. Downregulation of biglycan inhibits the proliferation of colon cancer cells and causes cell cycle arrest. (A) The effect of the downregulation of biglycan on HCT116 cell proliferation was measured using a cell counting kit‑8 assay, with each group containing six technical replicates. (B and C) The effect of the downregulation of biglycan on the HCT116 cell cycle distribution was analyzed by flow cytometry. (D) The protein expression levels of cyclin A, cyclin D1, p21 and p27 were detected by western blotting, and the results obtained from a representative experiment are shown. (E) The relative protein expres sion of these proteins were normalized against β‑actin. The data are expressed as the mean ± standard deviation (**P<0.01, compared with the shRNA‑control group). OD, optical density; sh, short hairpin.

Article Snippet: The membrane was subsequently blocked with 5% non-fat dry milk at room temperature for 1 h, followed by incubation with the following diluted primary antibodies: Rabbit polyclonal anti-biglycan antibody (cat no. sc-33788; 1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), rabbit polyclonal anti-caspase-3 antibody (cat no. wl01992a; 1:1,000), rabbit polyclonal anti-p27 antibody (cat no. wl01769; 1:1,000), rabbit polyclonal anti-cyclin A antibody (cat no. wl01753; 1:200), rabbit polyclonal anti-p21 antibody (cat no. wl0362; 1:100), rabbit polyclonal anti-cyclin D1 antibody (cat no. wl01435a; 1:100) (all from Wanleibio, Shenyang, China), rabbit polyclonal anti-p38 antibody (cat no. sc-7149; 1:100) and anti-phosphorylated-p38 antibody (cat no. sc-101758; 1:100) (both from Abcam, Cambridge, MA, USA) at 4 ̊C overnight.

Techniques: CCK-8 Assay, Flow Cytometry, Expressing, Western Blot, Standard Deviation

Journal: Molecular medicine reports

Article Title: Knockdown of biglycan expression by RNA interference inhibits the proliferation and invasion of, and induces apoptosis in, the HCT116 colon cancer cell line.

doi: 10.3892/mmr.2015.4383

Figure Lengend Snippet: Figure 3. Downregulation of biglycan suppresses the migratory and invasive properties of colon cancer cells (magnification, x200). (A) The motility of shRNA‑biglycan/control or non‑transfected cells was determined using a scratch wound assay over a 24 h time period. (B) The migration rate was determined by the distance traveled by the cells to the front of the denuded area. (C) The invasion ability of cells in each group was measured using a Transwell assay. (D) The number of invasive cells were counted under an inverted microscope and cell numbers were plotted. The results obtained from a representative experi ment are shown. The data are expressed as the mean ± standard deviation (**P<0.01, compared with the shRNA‑control group).. sh, short hairpin.

Article Snippet: The membrane was subsequently blocked with 5% non-fat dry milk at room temperature for 1 h, followed by incubation with the following diluted primary antibodies: Rabbit polyclonal anti-biglycan antibody (cat no. sc-33788; 1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), rabbit polyclonal anti-caspase-3 antibody (cat no. wl01992a; 1:1,000), rabbit polyclonal anti-p27 antibody (cat no. wl01769; 1:1,000), rabbit polyclonal anti-cyclin A antibody (cat no. wl01753; 1:200), rabbit polyclonal anti-p21 antibody (cat no. wl0362; 1:100), rabbit polyclonal anti-cyclin D1 antibody (cat no. wl01435a; 1:100) (all from Wanleibio, Shenyang, China), rabbit polyclonal anti-p38 antibody (cat no. sc-7149; 1:100) and anti-phosphorylated-p38 antibody (cat no. sc-101758; 1:100) (both from Abcam, Cambridge, MA, USA) at 4 ̊C overnight.

Techniques: Control, Scratch Wound Assay Assay, Migration, Transwell Assay, Inverted Microscopy, Standard Deviation

Journal: Molecular medicine reports

Article Title: Knockdown of biglycan expression by RNA interference inhibits the proliferation and invasion of, and induces apoptosis in, the HCT116 colon cancer cell line.

doi: 10.3892/mmr.2015.4383

Figure Lengend Snippet: Figure 4. Downregulation of biglycan activates the p38 signaling pathway and induces apoptosis in colon cancer cells. (A) The effect of biglycan downregula tion on the apoptosis of the HCT116 cells was detected using flow cytometry, and results are shown from a representative experiment. (B) The proportion of apoptotic cells in each group was determined. (C) The protein expression levels of caspase‑3 and p‑p38 were detected by western blotting, and the results obtained from a representative experiment are shown for each group. (D) The relative expression of these proteins was normalized against β‑actin. The data are expressed as the mean ± standard deviation (**P<0.01, compared with the shRNA‑control group; ##P<0.01, compared with the shRNA‑biglycan group). p‑, phosphorylated; sh, short hairpin.

Article Snippet: The membrane was subsequently blocked with 5% non-fat dry milk at room temperature for 1 h, followed by incubation with the following diluted primary antibodies: Rabbit polyclonal anti-biglycan antibody (cat no. sc-33788; 1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), rabbit polyclonal anti-caspase-3 antibody (cat no. wl01992a; 1:1,000), rabbit polyclonal anti-p27 antibody (cat no. wl01769; 1:1,000), rabbit polyclonal anti-cyclin A antibody (cat no. wl01753; 1:200), rabbit polyclonal anti-p21 antibody (cat no. wl0362; 1:100), rabbit polyclonal anti-cyclin D1 antibody (cat no. wl01435a; 1:100) (all from Wanleibio, Shenyang, China), rabbit polyclonal anti-p38 antibody (cat no. sc-7149; 1:100) and anti-phosphorylated-p38 antibody (cat no. sc-101758; 1:100) (both from Abcam, Cambridge, MA, USA) at 4 ̊C overnight.

Techniques: Flow Cytometry, Expressing, Western Blot, Standard Deviation